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(608) 263-9263
- Identification and characterization of botulinum neurotoxin-like two-component toxins in Paeniclostridium ghoniiPyung-Gang Lee, Linxiang Yin, Xin Wei, Jingyuan Shi, Geoffrey Masuyer, Travis G Wentz, Pengsheng Chen, Ying Xu, Junjie Liang, Haonan Zhang, Sara Persson Košenina, Briallen Lobb, Michael Mansfield, Sarjeet S Gill, Sabine Pellett, Pål Stenmark, Andrew C Doxey, Min Dong Science advances
- Botulinum neurotoxin Light Chain/A1 uses fast synaptic vesicle cycling to cleave plasma membrane bound SNAP-25Alexander Gardner, Molly Sneller, William H Tepp, Joseph T Barbieri, Sabine Pellett Communications biology
- Bifunctional Inhibition of Botulinum Neurotoxin A Protease: Unexpected Active Site Inhibition Enhances Covalent Targeting of an Allosteric SiteMingliang Lin, Nishant Karadkhelkar, Yanjie Li, Lisa M Eubanks, William H Tepp, Sabine Pellett, Kim D Janda Journal of medicinal chemistry
- Expression of Recombinant Clostridial Neurotoxin by C. tetaniBrieana M Gregg, Sonal Gupta, William H Tepp, Sabine Pellett Microorganisms
- Potency Evaluations of Recombinant Botulinum Neurotoxin A1 Mutants Designed to Reduce ToxicityPolrit Viravathana, William H Tepp, Marite Bradshaw, Amanda Przedpelski, Joseph T Barbieri, Sabine Pellett International journal of molecular sciences
- Botulinum neurotoxin X lacks potency in mice and in human neuronsBrieana M Gregg, Takuhiro Matsumura, Travis G Wentz, William H Tepp, Marite Bradshaw, Pål Stenmark, Eric A Johnson, Yukako Fujinaga, Sabine Pellett mBio
- Recent Developments in Vaccine Design: From Live Vaccines to Recombinant Toxin VaccinesSonal Gupta, Sabine Pellett Toxins
- Special Issue: Gram-Positive Bacterial ToxinsShashi Sharma, Sabine Pellett, Stephen A Morse Microorganisms
- Botulinum Neurotoxin A4 Has a 1000-Fold Reduced Potency Due to Three Single Amino Acid Alterations in the Protein Receptor Binding DomainWilliam H Tepp, Marite Bradshaw, Alexander P Gardner, Rebecca L Kaufman, Joseph T Barbieri, Sabine Pellett International journal of molecular sciences
- Transformation of a Metal Chelate into a "Catch and Anchor" Inhibitor of Botulinum A ProteaseLucy Lin, Ealin N Patel, Alexander L Nielsen, Lewis D Turner, William H Tepp, Kong Nguyen, Sabine Pellett, Kim Janda International journal of molecular sciences
- How Botulinum Neurotoxin Light Chain A1 Maintains Stable Association with the Intracellular Neuronal Plasma MembraneAlexander P Gardner, Joseph T Barbieri, Sabine Pellett Toxins
- Advances in Clostridial and Related NeurotoxinsSabine Pellett International journal of molecular sciences
- Genomic Diversity, Competition, and Toxin Production by Group I and II Clostridium botulinum Strains Used in Food Challenge StudiesBrooke Kathryn Bowe, Travis Gwynn Wentz, Brieana Marie Gregg, William Howard Tepp, Kristin Marie Schill, Shashi Sharma, Sabine Pellett Microorganisms
- Investigation of Salicylanilides as Botulinum Toxin AntagonistsEalin N Patel, Lucy Lin, Molly M Sneller, Lisa M Eubanks, William H Tepp, Sabine Pellett, Kim D Janda ACS infectious diseases
- Preclinical Evidence for the Role of Botulinum Neurotoxin A (BoNT/A) in the Treatment of Peripheral Nerve InjuryMichael Adler, Sabine Pellett, Shashi K Sharma, Frank J Lebeda, Zygmunt F Dembek, Mark A Mahan Microorganisms
- Endogenous CRISPR-Cas Systems in Group I Clostridium botulinum and Clostridium sporogenes Do Not Directly Target the Botulinum Neurotoxin Gene ClusterTravis G Wentz, Benjamin J M Tremblay, Marite Bradshaw, Andrew C Doxey, Shashi K Sharma, John-Demian Sauer, Sabine Pellett Frontiers in microbiology
- Resolution of Two Steps in Botulinum Neurotoxin Serotype A1 Light Chain Localization to the Intracellular Plasma MembraneAlexander Gardner, William H Tepp, Marite Bradshaw, Joseph T Barbieri, Sabine Pellett International journal of molecular sciences
- Posttranslational Regulation of Botulinum Neurotoxin Production in Clostridium botulinum Hall A-hyperHeather N'te Inzalaco, William H Tepp, Chase Fredrick, Marite Bradshaw, Eric A Johnson, Sabine Pellett mSphere
- Irreversible inhibition of BoNT/A protease: proximity-driven reactivity contingent upon a bifunctional approachLewis D Turner, Alexander L Nielsen, Lucy Lin, Sabine Pellett, Takashi Sugane, Margaret E Olson, Eric A Johnson, Kim D Janda RSC medicinal chemistry
- COVALENT INHIBITION OF BOTULINUM NEUROTOXIN A - EXPLORATION OF WARHEAD REACTIVITY AND FUNCTION USING A BIFUNCTIONAL APPROACHLewis D Turner, Alexander L Nielsen, Lucy Lin, Sabine Pellett, Takashi Sugane, Margaret E Olson, Kim D Janda Toxicon : official journal of the International Society on Toxinology
- Identification of 3-hydroxy-1,2-dimethylpyridine-4(1H)-thione as a metal-binding motif for the inhibition of botulinum neurotoxin ALucy Lin, Lewis D Turner, Peter Šilhár, Sabine Pellett, Eric A Johnson, Kim D Janda RSC medicinal chemistry
- Catch and Anchor Approach To Combat Both Toxicity and Longevity of Botulinum Toxin ALucy Lin, Margaret E Olson, Takashi Sugane, Lewis D Turner, Margarita A Tararina, Alexander L Nielsen, Elbek K Kurbanov, Sabine Pellett, Eric A Johnson, Seth M Cohen, Karen N Allen, Kim D Janda Journal of medicinal chemistry
- A Novel High-Potency Tetanus VaccineAmanda Przedpelski, William H Tepp, Sabine Pellett, Eric A Johnson, Joseph T Barbieri mBio
- Critical Analysis of Neuronal Cell and the Mouse Bioassay for Detection of Botulinum NeurotoxinsSabine Pellett, William H Tepp, Eric A Johnson Toxins
- Botulinum neurotoxins A, B, C, E, and F preferentially enter cultured human motor neurons compared to other cultured human neuronal populationsSabine Pellett, William H Tepp, Eric A Johnson FEBS letters
- Comparative functional analysis of mice after local injection with botulinum neurotoxin A1, A2, A6, and B1 by catwalk analysisMolly S Moritz, William H Tepp, Heather N'te Inzalaco, Eric A Johnson, Sabine Pellett Toxicon : official journal of the International Society on Toxinology
- Isolation and Characterization of the Novel Botulinum Neurotoxin A Subtype 6Molly S Moritz, William H Tepp, Marite Bradshaw, Eric A Johnson, Sabine Pellett mSphere
- The Light Chain Defines the Duration of Action of Botulinum Toxin Serotype A SubtypesSabine Pellett, Marite Bradshaw, William H Tepp, Christina L Pier, Regina C M Whitemarsh, Chen Chen, Joseph T Barbieri, Eric A Johnson mBio
- A Study and Review of Effects of Botulinum Toxins on Mast Cell Dependent and Independent PruritusRoshni Ramachandran, Marc J Marino, Snighdha Paul, Zhenping Wang, Nicholas L Mascarenhas, Sabine Pellett, Eric A Johnson, Anna DiNardo, Tony L Yaksh Toxins
- Substrate cleavage and duration of action of botulinum neurotoxin type FA ("H, HA")Sabine Pellett, William H Tepp, Guangyun Lin, Eric A Johnson Toxicon : official journal of the International Society on Toxinology
- Small molecule metalloprotease inhibitor with in vitro, ex vivo and in vivo efficacy against botulinum neurotoxin serotype AAlan R Jacobson, Michael Adler, Nicholas R Silvaggi, Karen N Allen, Genessa M Smith, Ross A Fredenburg, Ross L Stein, Jong-Beak Park, Xiaochuan Feng, Charles B Shoemaker, Sharad S Deshpande, Michael C Goodnough, Carl J Malizio, Eric A Johnson, Sabine Pellett, William H Tepp, Saul Tzipori Toxicon : official journal of the International Society on Toxinology
- Metal Ions Effectively Ablate the Action of Botulinum Neurotoxin APaul T Bremer, Sabine Pellett, James P Carolan, William H Tepp, Lisa M Eubanks, Karen N Allen, Eric A Johnson, Kim D Janda Journal of the American Chemical Society
- Assessment of ELISA as endpoint in neuronal cell-based assay for BoNT detection using hiPSC derived neuronsSabine Pellett, William H Tepp, Eric A Johnson, Dorothea Sesardic Journal of pharmacological and toxicological methods
- Purification and Characterization of Botulinum Neurotoxin FA from a Genetically Modified Clostridium botulinum StrainSabine Pellett, William H Tepp, Marite Bradshaw, Suzanne R Kalb, Janet K Dykes, Guangyun Lin, Erin M Nawrocki, Christina L Pier, John R Barr, Susan E Maslanka, Eric A Johnson mSphere
- Cellular Protection of SNAP-25 against Botulinum Neurotoxin/A: Inhibition of Thioredoxin Reductase through a Suicide Substrate MechanismHajime Seki, Song Xue, Sabine Pellett, Peter Šilhár, Eric A Johnson, Kim D Janda Journal of the American Chemical Society
- Current status and future directions of botulinum neurotoxins for targeting pain processingSabine Pellett, Tony L Yaksh, Roshni Ramachandran Toxins
- Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum NeurotoxinSabine Pellett, Michael P Schwartz, William H Tepp, Richard Josephson, Jacob M Scherf, Christina L Pier, James A Thomson, William L Murphy, Eric A Johnson Scientific reports
- Heat-Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into NeuronsChen Chen, Amanda Przedpelski, William H Tepp, Sabine Pellett, Eric A Johnson, Joseph T Barbieri mBio
- Botulinum Neurotoxins Can Enter Cultured Neurons Independent of Synaptic Vesicle RecyclingSabine Pellett, William H Tepp, Jacob M Scherf, Eric A Johnson PloS one
- In vivo onset and duration of action varies for botulinum neurotoxin A subtypes 1-5Sabine Pellett, William H Tepp, Regina C M Whitemarsh, Marite Bradshaw, Eric A Johnson Toxicon : official journal of the International Society on Toxinology
- A Novel Botulinum Neurotoxin, Previously Reported as Serotype H, Has a Hybrid-Like Structure With Regions of Similarity to the Structures of Serotypes A and F and Is Neutralized With Serotype A AntitoxinSusan E Maslanka, Carolina Lúquez, Janet K Dykes, William H Tepp, Christina L Pier, Sabine Pellett, Brian H Raphael, Suzanne R Kalb, John R Barr, Agam Rao, Eric A Johnson The Journal of infectious diseases
- Activity of botulinum neurotoxin type D (strain 1873) in human neuronsSabine Pellett, William H Tepp, Jacob M Scherf, Christina L Pier, Eric A Johnson Toxicon : official journal of the International Society on Toxinology
- Toward the discovery of dual inhibitors for botulinum neurotoxin A: concomitant targeting of endocytosis and light chain protease activityHajime Seki, Song Xue, Mark S Hixon, Sabine Pellett, Marek Remes, Eric A Johnson, Kim D Janda Chemical communications (Cambridge, England)
- Analysis of gene expression in induced pluripotent stem cell-derived human neurons exposed to botulinum neurotoxin A subtype 1 and a type A atoxic derivativeJacob M Scherf, Xiaoyang Serene Hu, William H Tepp, Konstantin Ichtchenko, Eric A Johnson, Sabine Pellett PloS one
- Holotoxin Activity of Botulinum Neurotoxin Subtype A4 Originating from a Nontoxigenic Clostridium botulinum Expression SystemMarite Bradshaw, William H Tepp, Regina C M Whitemarsh, Sabine Pellett, Eric A Johnson Applied and environmental microbiology
- Persistence of botulinum neurotoxin a subtypes 1-5 in primary rat spinal cord cellsRegina Clare Meyer Whitemarsh, William Howard Tepp, Eric Arthur Johnson, Sabine Pellett PloS one
- Synthesis/biological evaluation of hydroxamic acids and their prodrugs as inhibitors for Botulinum neurotoxin A light chainHajime Seki, Sabine Pellett, Peter Silhár, G Neil Stowe, Beatriz Blanco, Matthew A Lardy, Eric A Johnson, Kim D Janda Bioorganic & medicinal chemistry
- Targeting botulinum A cellular toxicity: a prodrug approachPeter Silhár, Lisa M Eubanks, Hajime Seki, Sabine Pellett, Sacha Javor, William H Tepp, Eric A Johnson, Kim D Janda Journal of medicinal chemistry
- Comparison of the catalytic properties of the botulinum neurotoxin subtypes A1 and A5Dongxia Wang, Joan Krilich, Sabine Pellett, Jakub Baudys, William H Tepp, John R Barr, Eric A Johnson, Suzanne R Kalb Biochimica et biophysica acta
- Characterization of botulinum neurotoxin A subtypes 1 through 5 by investigation of activities in mice, in neuronal cell cultures, and in vitroRegina C M Whitemarsh, William H Tepp, Marite Bradshaw, Guangyun Lin, Christina L Pier, Jacob M Scherf, Eric A Johnson, Sabine Pellett Infection and immunity
- Evaluation of adamantane hydroxamates as botulinum neurotoxin inhibitors: synthesis, crystallography, modeling, kinetic and cellular based studiesPeter Šilhár, Nicholas R Silvaggi, Sabine Pellett, Kateřina Čapková, Eric A Johnson, Karen N Allen, Kim D Janda Bioorganic & medicinal chemistry
- Progress in cell based assays for botulinum neurotoxin detectionSabine Pellett Current topics in microbiology and immunology
- Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cellsRegina C M Whitemarsh, Christina L Pier, William H Tepp, Sabine Pellett, Eric A Johnson Biochemical and biophysical research communications
- Learning from the past: historical aspects of bacterial toxins as pharmaceuticalsSabine Pellett Current opinion in microbiology
- Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detectionRegina C M Whitemarsh, Monica J Strathman, Lucas G Chase, Casey Stankewicz, William H Tepp, Eric A Johnson, Sabine Pellett Toxicological sciences : an official journal of the Society of Toxicology
- Rapid immune responses to a botulinum neurotoxin Hc subunit vaccine through in vivo targeting to antigen-presenting cellsDavid M White, Sabine Pellett, Mark A Jensen, William H Tepp, Eric A Johnson, Barry G W Arnason Infection and immunity
- Neuronal targeting, internalization, and biological activity of a recombinant atoxic derivative of botulinum neurotoxin ASabine Pellett, William H Tepp, Larry H Stanker, Philip A Band, Eric A Johnson, Konstantin Ichtchenko Biochemical and biophysical research communications
- Sensitive and quantitative detection of botulinum neurotoxin in neurons derived from mouse embryonic stem cellsSabine Pellett, Zhong-wei Du, Christina L Pier, William H Tepp, Su-chun Zhang, Eric A Johnson Biochemical and biophysical research communications
- Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1Christina L Pier, Chen Chen, William H Tepp, Guangyun Lin, Kim D Janda, Joseph T Barbieri, Sabine Pellett, Eric A Johnson FEBS letters
- Comparison of the primary rat spinal cord cell (RSC) assay and the mouse bioassay for botulinum neurotoxin type A potency determinationSabine Pellett, William H Tepp, Stephen I Toth, Eric A Johnson Journal of pharmacological and toxicological methods
- Toosendanin: synthesis of the AB-ring and investigations of its anti-botulinum properties (Part II)Yuya Nakai, Sabine Pellett, William H Tepp, Eric A Johnson, Kim D Janda Bioorganic & medicinal chemistry
- Benzylidene cyclopentenediones: First irreversible inhibitors against botulinum neurotoxin A's zinc endopeptidaseKaterina Capková, Mark S Hixon, Sabine Pellett, Joseph T Barbieri, Eric A Johnson, Kim D Janda Bioorganic & medicinal chemistry letters
- Bimodal modulation of the botulinum neurotoxin protein-conducting channelAudrey Fischer, Yuya Nakai, Lisa M Eubanks, Colin M Clancy, William H Tepp, Sabine Pellett, Tobin J Dickerson, Eric A Johnson, Kim D Janda, Mauricio Montal Proceedings of the National Academy of Sciences of the United States of America
- Selection and characterization of a human monoclonal neutralizing antibody for Clostridium Botulinum neurotoxin serotype BHeyue Zhou, Bin Zhou, Sabine Pellett, Eric A Johnson, Kim D Janda Bioorganic & medicinal chemistry letters
- Delineating the susceptibility of botulinum neurotoxins to denaturation through thermal effectsBin Zhou, Sabine Pellett, William H Tepp, Heyue Zhou, Eric A Johnson, Kim D Janda FEBS letters
- A neuronal cell-based botulinum neurotoxin assay for highly sensitive and specific detection of neutralizing serum antibodiesSabine Pellett, William H Tepp, Colin M Clancy, Gary E Borodic, Eric A Johnson FEBS letters
- Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypesKristin M Marshall, Marite Bradshaw, Sabine Pellett, Eric A Johnson Biochemical and biophysical research communications
- Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeast Saccharomyces cerevisiaeSabine Pellett, James W Tracy Yeast (Chichester, England)
Our lab’s research focuses on Clostridium botulinum (C. botulinum) and its neurotoxins as pathogens and agents of serious foodborne illness and as important and unique pharmaceuticals that are widely used for many clinical applications around the world. We are a state-of-the-art lab continuing a long-term botulinum neurotoxins (BoNTs) research program with a rich history at UW-Madison, starting with Ed Schantz’s production of first crystalline BoNTs, Ed Schantz and Eric Johnson’s production of the first batches of pharmaceutically used BoNTs, and many novel discoveries.
Ongoing research in our lab examines toxin production by C. botulinum, purification and characterization of BoNTs and related homologs, lateral transfer of BoNT genes within Clostridium, structure function studies of novel BoNTs, investigations of countermeasures for botulism including vaccines and small molecule inhibitors, development of novel BoNT based pharmaceuticals, tetanus vaccines, and food safety studies.
Education
B.S. Biology, University of Heidelberg, Heidelberg, Germany
M.A. Biology, Portland State University, Portland, OR
PhD. Env. & Mol. Toxicology, University of Wisconsin-Madison
Postdoctoral Research: Food Microbiology & Toxicology, University of Wisconsin-Madison
Affiliations
Member of International Neurotoxin Association: 2012 – current
Member of American Society of Microbiologists (ASM): 2013-current
Consultant for Merz Pharma (Germany): 2013 – ongoing
Expert consultant for UW-Madison Institutional Biosafety Committee: 2017-current