Faculty & Staff

  • Image of Jorge Escalante

    Jorge Escalante

    Professor of Bacteriology, Emeritus
    Office: (706) 542-2651
    jcescala@uga.edu

Education

M.S. 1979 University of Illinois-Urbana
Ph.D. 1983 University of Illinois-Urbana
Postdoctoral Research: University of Illinois-Urbana; University of Utah-Salt Lake City

Areas of Study

Prokaryotic metabolism and physiology.

Research Papers

  • Newmister SA, Chan CH, Escalante-Semerena JC, Rayment I (2012) Structural insights into the function of the nicotinate mononucleotide:phenol/p-cresol phosphoribosyltransferase (ArsAB) enzyme from Sporomusa ovata. Biochemistry 51(43):8571-82 · Pubmed

    No abstract available.

  • Thao S, Escalante-Semerena JC (2012) A positive selection approach identifies residues important for folding of Salmonella enterica Pat, an N(Χζ)-lysine acetyltransferase that regulates central metabolism enzymes. Res. Microbiol. 163(7-Jun):427-35 PMC3432723 · Pubmed

    No abstract available.

  • Crosby HA, Rank KC, Rayment I, Escalante-Semerena JC (2012) Structure-Guided Expansion of the Substrate Range of Methylmalonyl Coenzyme A Synthetase (MatB) of Rhodopseudomonas palustris. Appl. Environ. Microbiol. 78(18):6619-29 PMC3426712 · Pubmed

    No abstract available.

  • Stuecker TN, Tucker AC, Escalante-Semerena JC (2012) PanM, an acetyl-coenzyme A sensor required for maturation of L-aspartate decarboxylase (PanD). MBio 3(4): PMC3398536 · Pubmed

    No abstract available.

  • Crosby HA, Pelletier DA, Hurst GB, Escalante-Semerena JC (2012) System-wide studies of N-lysine acetylation in Rhodopseudomonas palustris reveal substrate specificity of protein acetyltransferases. J. Biol. Chem. 287(19):15590-601 PMC3346091 [Available on 05/04/13] · Pubmed

    No abstract available.

  • Stuecker TN, Hodge KM, Escalante-Semerena JC (2012) The missing link in coenzyme A biosynthesis: PanM (formerly YhhK), a yeast GCN5 acetyltransferase homologue triggers aspartate decarboxylase (PanD) maturation in Salmonella enterica. Mol. Microbiol. 84(4):608-19 PMC3345047 [Available on 05/01/13] · Pubmed

    No abstract available.

  • Park K, Mera PE, Escalante-Semerena JC, Brunold TC (2012) Spectroscopic characterization of active-site variants of the PduO-type ATP:corrinoid adenosyltransferase from Lactobacillus reuteri: insights into the mechanism of four-coordinate Co(II)corrinoid formation. Inorg Chem 51(8):4482-94 · Pubmed

    No abstract available.

  • Thao S, Escalante-Semerena JC (2011) Biochemical and thermodynamic analyses of Salmonella enterica Pat, a multidomain, multimeric N(Χζ)-lysine acetyltransferase involved in carbon and energy metabolism. MBio 2(5): PMC3195501 · Pubmed

    No abstract available.

  • Chan CH, Escalante-Semerena JC (2011) ArsAB, a novel enzyme from Sporomusa ovata activates phenolic bases for adenosylcobamide biosynthesis. Mol. Microbiol. 81(4):952-67 PMC3182833 · Pubmed

    No abstract available.

  • Newmister SA, Otte MM, Escalante-Semerena JC, Rayment I (2011) Structure and mutational analysis of the archaeal GTP:AdoCbi-P guanylyltransferase (CobY) from Methanocaldococcus jannaschii: insights into GTP binding and dimerization. Biochemistry 50(23):5301-13 PMC3125946 · Pubmed

    No abstract available.

  • Thao S, Escalante-Semerena JC (2011) Control of protein function by reversible N_-lysine acetylation in bacteria. Curr. Opin. Microbiol. 14(2):200-4 PMC3078959 · Pubmed

    No abstract available.

  • Chan CH, Garrity J, Crosby HA, Escalante-Semerena JC (2011) In Salmonella enterica, the sirtuin-dependent protein acylation/deacylation system (SDPADS) maintains energy homeostasis during growth on low concentrations of acetate. Mol. Microbiol. 80(1):168-83 · Pubmed

    No abstract available.

  • Bheda P, Wang JT, Escalante-Semerena JC, Wolberger C (2011) Structure of Sir2Tm bound to a propionylated peptide. Protein Sci. 20(1):131-139 PMC3047069 · Pubmed

    No abstract available.

  • Thao S, Chen CS, Zhu H, Escalante-Semerena JC (2010) NΧζ-lysine acetylation of a bacterial transcription factor inhibits Its DNA-binding activity. PLoS ONE 5(12):e15123 PMC3013089 · Pubmed

    No abstract available.

  • Tucker AC, Escalante-Semerena JC (2010) Biologically active isoforms of CobB sirtuin deacetylase in Salmonella enterica and Erwinia amylovora. J. Bacteriol. 192(23):6200-8 PMC2981217 · Pubmed

    No abstract available.

  • Gray MJ, Escalante-Semerena JC (2010) A new pathway for the synthesis of Χρ-ribazole-phosphate in Listeria innocua. Mol. Microbiol. 77(6):1429-38 PMC2948856 · Pubmed

    No abstract available.

  • Mera PE, Escalante-Semerena JC (2010) Multiple roles of ATP:cob(I)alamin adenosyltransferases in the conversion of B12 to coenzyme B12. Appl. Microbiol. Biotechnol. 88(1):41-8 PMC3034633 · Pubmed

    No abstract available.

  • Crosby HA, Heiniger EK, Harwood CS, Escalante-Semerena JC (2010) Reversible N epsilon-lysine acetylation regulates the activity of acyl-CoA synthetases involved in anaerobic benzoate catabolism in Rhodopseudomonas palustris. Mol. Microbiol. 76(4):874-88 PMC2913386 · Pubmed

    No abstract available.

  • Claas KR, Parrish JR, Maggio-Hall LA, Escalante-Semerena JC (2010) Functional analysis of the nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) enzyme, involved in the late steps of coenzyme B12 biosynthesis in Salmonella enterica. J. Bacteriol. 192(1):145-154 PMC2798273 · Pubmed

    No abstract available.

  • Mera PE, Escalante-Semerena JC (2010) Dihydroflavin-driven adenosylation of 4-coordinate Co(II) corrinoids: are cobalamin reductases enzymes or electron transfer proteins? J. Biol. Chem. 285(5):2911-2917 PMC2823439 · Pubmed

    No abstract available.

  • Rocco CJ, Escalante-Semerena JC (2010) In Salmonella enterica, 2-methylcitrate blocks gluconeogenesis. J. Bacteriol. 192(3):771-778 PMC2812455 · Pubmed

    No abstract available.

  • Gray MJ, Escalante-Semerena JC (2009) The cobinamide amidohydrolase (cobyric acid-forming) CbiZ enzyme: a critical activity of the cobamide remodelling system of Rhodobacter sphaeroides. Mol. Microbiol. 74(5):1198-210 PMC3062942 · Pubmed

    No abstract available.

  • Otte MM, Escalante-Semerena JC (2009) Biochemical characterization of the GTP:adenosylcobinamide-phosphate guanylyltransferase (CobY) enzyme of the hyperthermophilic archaeon Methanocaldococcus jannaschii. Biochemistry 48(25):5882-5889 PMC2757067 · Pubmed

    No abstract available.

  • Lewis JA, Stamper LW, Escalante-Semerena JC (2009) Regulation of expression of the tricarballylate utilization operon (tcuABC) of Salmonella enterica. Res. Microbiol. 160(3):179-186 PMC2692759 · Pubmed

    No abstract available.

  • Gray MJ, Escalante-Semerena JC (2009) In vivo analysis of cobinamide salvaging in Rhodobacter sphaeroides strain 2.4.1. J. Bacteriol. 191(12):3842-3851 PMC2698395 · Pubmed

    No abstract available.

  • Mera PE, St Maurice M, Rayment I, Escalante-Semerena JC (2009) Residue Phe112 of the human-type corrinoid adenosyltransferase (PduO) enzyme of Lactobacillus reuteri is critical to the formation of the four-coordinate Co(II) corrinoid substrate and to the activity of the enzyme. Biochemistry 48(14):3138-3145 PMC2666787 · Pubmed

    No abstract available.

  • Lewis JA, Boyd JM, Downs DM, Escalante-Semerena JC (2009) Involvement of the Cra global regulatory protein in the expression of the iscRSUA operon, revealed during studies of tricarballylate catabolism in Salmonella enterica. J. Bacteriol. 191(7):2069-2076 PMC2655522 · Pubmed

    No abstract available.

  • Gardner JG, Escalante-Semerena JC (2009) In Bacillus subtilis, the sirtuin protein deacetylase, encoded by the srtN gene (formerly yhdZ), and functions encoded by the acuABC genes control the activity of acetyl coenzyme A synthetase. J. Bacteriol. 191(6):1749-1755 PMC2648355 · Pubmed

    No abstract available.

  • Gray MJ, Tavares NK, Escalante-Semerena JC (2008) The genome of Rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins. Mol. Microbiol. 70(4):824-836 PMC2602876 · Pubmed

    No abstract available.

  • Park K, Mera PE, Escalante-Semerena JC, Brunold TC (2008) Kinetic and spectroscopic studies of the ATP:corrinoid adenosyltransferase PduO from Lactobacillus reuteri: substrate specificity and insights into the mechanism of Co(II)corrinoid reduction. Biochemistry 47(34):9007-9015 PMC2574751 · Pubmed

    No abstract available.

  • Gardner JG, Escalante-Semerena JC (2008) Biochemical and mutational analyses of AcuA, the acetyltransferase enzyme that controls the activity of the acetyl coenzyme a synthetase (AcsA) in Bacillus subtilis. J. Bacteriol. 190(14):5132-5136 PMC2446994 · Pubmed

    No abstract available.

  • Boyd JM, Lewis JA, Escalante-Semerena JC, Downs DM (2008) Salmonella enterica requires ApbC function for growth on tricarballylate: evidence of functional redundancy between ApbC and IscU. J. Bacteriol. 190(13):4596-4602 PMC2446783 · Pubmed

    No abstract available.

  • St Maurice M, Mera P, Park K, Brunold TC, Escalante-Semerena JC, Rayment I (2008) Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate intermediate. Biochemistry 47(21):5755-5766 · Pubmed

    No abstract available.

  • Rocco CJ, Dennison KL, Klenchin VA, Rayment I, Escalante-Semerena JC (2008) Construction and use of new cloning vectors for the rapid isolation of recombinant proteins from Escherichia coli. Plasmid 59(3):231-237 PMC2386272 · Pubmed

    No abstract available.

  • Ruiz-SΗ­nchez P, Mundwiler S, Spingler B, Buan NR, Escalante-Semerena JC, Alberto R (2008) Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymatic assay. J. Biol. Inorg. Chem. 13(3):335-347 · Pubmed

    No abstract available.

  • Mera PE, St Maurice M, Rayment I, Escalante-Semerena JC (2007) Structural and functional analyses of the human-type corrinoid adenosyltransferase (PduO) from Lactobacillus reuteri. Biochemistry 46(48):13829-13836 · Pubmed

    No abstract available.

  • Zayas CL, Claas K, Escalante-Semerena JC (2007) The CbiB protein of Salmonella enterica is an integral membrane protein involved in the last step of the de novo corrin ring biosynthetic pathway. J. Bacteriol. 189(21):7697-7708 PMC2168724 · Pubmed

    No abstract available.

  • Garrity J, Gardner JG, Hawse W, Wolberger C, Escalante-Semerena JC (2007) N-lysine propionylation controls the activity of propionyl-CoA synthetase. J. Biol. Chem. 282(41):30239-30245 · Pubmed

    No abstract available.

  • Otte MM, Woodson JD, Escalante-Semerena JC (2007) The thiamine kinase (YcfN) enzyme plays a minor but significant role in cobinamide salvaging in Salmonella enterica. J. Bacteriol. 189(20):7310-7315 PMC2168424 · Pubmed

    No abstract available.

  • Lewis JA, Escalante-Semerena JC (2007) Tricarballylate catabolism in Salmonella enterica. The TcuB protein uses 4Fe-4S clusters and heme to transfer electrons from FADH2 in the tricarballylate dehydrogenase (TcuA) enzyme to electron acceptors in the cell membrane. Biochemistry 46(31):9107-9115 · Pubmed

    No abstract available.

  • Garvey GS, Rocco CJ, Escalante-Semerena JC, Rayment I (2007) The three-dimensional crystal structure of the PrpF protein of Shewanella oneidensis complexed with trans-aconitate: insights into its biological function. Protein Sci. 16(7):1274-1284 PMC2206708 · Pubmed

    No abstract available.

  • Escalante-Semerena JC (2007) Conversion of cobinamide into adenosylcobamide in bacteria and archaea. J. Bacteriol. 189(13):4555-4560 PMC1913469 · Pubmed

    No abstract available.

  • Brinsmade SR, Escalante-Semerena JC (2007) In vivo and in vitro analyses of single-amino acid variants of the Salmonella enterica phosphotransacetylase enzyme provide insights into the function of its N-terminal domain. J. Biol. Chem. 282(17):12629-12640 · Pubmed

    No abstract available.

  • Zayas CL, Escalante-Semerena JC (2007) Reassessment of the late steps of coenzyme B12 synthesis in Salmonella enterica: evidence that dephosphorylation of adenosylcobalamin-5'-phosphate by the CobC phosphatase is the last step of the pathway. J. Bacteriol. 189(6):2210-2218 PMC1899380 · Pubmed

    No abstract available.

  • Gray MJ, Escalante-Semerena JC (2007) Single-enzyme conversion of FMNH2 to 5,6-dimethylbenzimidazole, the lower ligand of B12. Proc. Natl. Acad. Sci. U.S.A. 104(8):2921-2916 PMC1815282 · Pubmed

    No abstract available.

  • St Maurice M, Mera PE, Taranto MP, Sesma F, Escalante-Semerena JC, Rayment I (2007) Structural characterization of the active site of the PduO-type ATP:Co(I)rrinoid adenosyltransferase from Lactobacillus reuteri. J. Biol. Chem. 282(4):2596-2605 · Pubmed

    No abstract available.

  • Gardner JG, Grundy FJ, Henkin TM, Escalante-Semerena JC (2006) Control of acetyl-coenzyme A synthetase (AcsA) activity by acetylation/deacetylation without NAD(+) involvement in Bacillus subtilis. J. Bacteriol. 188(15):5460-5468 PMC1540023 · Pubmed

    No abstract available.

  • Lewis JA, Escalante-Semerena JC (2006) The FAD-dependent tricarballylate dehydrogenase (TcuA) enzyme of Salmonella enterica converts tricarballylate into cis-aconitate. J. Bacteriol. 188(15):5479-5486 PMC1540016 · Pubmed

    No abstract available.

  • Buan NR, Escalante-Semerena JC (2006) Purification and initial biochemical characterization of ATP:Cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica. J. Biol. Chem. 281(25):16971-16977 · Pubmed

    No abstract available.

  • Woodson JD, Escalante-Semerena JC (2006) The cbiS gene of the archaeon Methanopyrus kandleri AV19 encodes a bifunctional enzyme with adenosylcobinamide amidohydrolase and alpha-ribazole-phosphate phosphatase activities. J. Bacteriol. 188(12):4227-4235 PMC1482944 · Pubmed

    No abstract available.

  • Buan NR, Rehfeld K, Escalante-Semerena JC (2006) Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1. J. Bacteriol. 188(10):3543-50 PMC1482872 · Pubmed

    No abstract available.

  • Zayas CL, Woodson JD, Escalante-Semerena JC (2006) The cobZ gene of Methanosarcina mazei Go1 encodes the nonorthologous replacement of the alpha-ribazole-5'-phosphate phosphatase (CobC) enzyme of Salmonella enterica. J. Bacteriol. 188(7):2740-2743 PMC1428423 · Pubmed

    No abstract available.

  • Buan NR, Escalante-Semerena JC (2005) Computer-assisted docking of flavodoxin with the ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme reveals residues critical for protein-protein interactions but not for catalysis. J. Biol. Chem. 280(49):40948-40956 · Pubmed

    No abstract available.

  • Brinsmade SR, Paldon T, Escalante-Semerena JC (2005) Minimal functions and physiological conditions required for growth of salmonella enterica on ethanolamine in the absence of the metabolosome. J. Bacteriol. 187(23):8039-8046 PMC1291257 · Pubmed

    No abstract available.

  • Starai VJ, Garrity J, Escalante-Semerena JC (2005) Acetate excretion during growth of Salmonella enterica on ethanolamine requires phosphotransacetylase (EutD) activity, and acetate recapture requires acetyl-CoA synthetase (Acs) and phosphotransacetylase (Pta) activities. Microbiology (Reading, Engl.) 151(Pt 11):3793-3801 · Pubmed

    No abstract available.

  • Woodson JD, Reynolds AA, Escalante-Semerena JC (2005) ABC transporter for corrinoids in Halobacterium sp. strain NRC-1. J. Bacteriol. 187(17):5901-5909 PMC1196138 · Pubmed

    No abstract available.

  • Starai VJ, Gardner JG, Escalante-Semerena JC (2005) Residue Leu-641 of Acetyl-CoA synthetase is critical for the acetylation of residue Lys-609 by the Protein acetyltransferase enzyme of Salmonella enterica. J. Biol. Chem. 280(28):26200-26205 · Pubmed

    No abstract available.

  • Stich TA, Buan NR, Escalante-Semerena JC, Brunold TC (2005) Spectroscopic and computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis. J. Am. Chem. Soc. 127(24):8710-8719 · Pubmed

    No abstract available.

  • Palacios S, Escalante-Semerena JC (2004) 2-Methylcitrate-dependent activation of the propionate catabolic operon (prpBCDE) of Salmonella enterica by the PrpR protein. Microbiology (Reading, Engl.) 150(Pt 11):3877-3887 · Pubmed

    No abstract available.

  • Starai VJ, Escalante-Semerena JC (2004) Acetyl-coenzyme A synthetase (AMP forming). Cell. Mol. Life Sci. 61(16):2020-30 · Pubmed

    No abstract available.

  • Buan NR, Suh SJ, Escalante-Semerena JC (2004) The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme. J. Bacteriol. 186(17):5708-5714 PMC516830 · Pubmed

    No abstract available.

  • Starai VJ, Escalante-Semerena JC (2004) Identification of the protein acetyltransferase (Pat) enzyme that acetylates acetyl-CoA synthetase in Salmonella enterica. J. Mol. Biol. 340(5):1005-12 · Pubmed

    No abstract available.

  • Maggio-Hall LA, Claas KR, Escalante-Semerena JC (2004) The last step in coenzyme B(12) synthesis is localized to the cell membrane in bacteria and archaea. Microbiology (Reading, Engl.) 150(Pt 5):1385-1395 · Pubmed

    No abstract available.

  • Starai VJ, Takahashi H, Boeke JD, Escalante-Semerena JC (2004) A link between transcription and intermediary metabolism: a role for Sir2 in the control of acetyl-coenzyme A synthetase. Curr. Opin. Microbiol. 7(2):115-119 · Pubmed

    No abstract available.

  • Woodson JD, Escalante-Semerena JC (2004) CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea. Proc. Natl. Acad. Sci. U.S.A. 101(10):3591-6 PMC373507 · Pubmed

    No abstract available.

  • Lewis JA, Horswill AR, Schwem BE, Escalante-Semerena JC (2004) The Tricarballylate utilization (tcuRABC) genes of Salmonella enterica serovar Typhimurium LT2. J. Bacteriol. 186(6):1629-1637 PMC355976 · Pubmed

    No abstract available.

  • Brinsmade SR, Escalante-Semerena JC (2004) The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity. J. Bacteriol. 186(6):1890-2 PMC355984 · Pubmed

    No abstract available.

  • Grimek TL, Escalante-Semerena JC (2004) The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo. J. Bacteriol. 186(2):454-462 PMC305763 · Pubmed

    No abstract available.

  • Woodson JD, Zayas CL, Escalante-Semerena JC (2003) A new pathway for salvaging the coenzyme B12 precursor cobinamide in archaea requires cobinamide-phosphate synthase (CbiB) enzyme activity. J. Bacteriol. 185(24):7193-7201 PMC296239 · Pubmed

    No abstract available.

  • Grimek TL, Holden H, Rayment I, Escalante-Semerena JC (2003) Residues C123 and D58 of the 2-methylisocitrate lyase (PrpB) enzyme of Salmonella enterica are essential for catalysis. J. Bacteriol. 185(16):4837-4843 PMC166468 · Pubmed

    No abstract available.

  • Maggio-Hall LA, Dorrestein PC, Escalante-Semerena JC, Begley TP (2003) Formation of the dimethylbenzimidazole ligand of coenzyme B(12) under physiological conditions by a facile oxidative cascade. Org. Lett. 5(13):2211-2213 · Pubmed

    No abstract available.

  • Maggio-Hall LA, Escalante-Semerena JC (2003) Alpha-5,6-dimethylbenzimidazole adenine dinucleotide (alpha-DAD), a putative new intermediate of coenzyme B12 biosynthesis in Salmonella typhimurium. Microbiology (Reading, Engl.) 149(Pt 4):983-990 · Pubmed

    No abstract available.

  • Palacios S, Starai VJ, Escalante-Semerena JC (2003) Propionyl coenzyme A is a common intermediate in the 1,2-propanediol and propionate catabolic pathways needed for expression of the prpBCDE operon during growth of Salmonella enterica on 1,2-propanediol. J. Bacteriol. 185(9):2802-2810 PMC154405 · Pubmed

    No abstract available.

  • Gulick AM, Starai VJ, Horswill AR, Homick KM, Escalante-Semerena JC (2003) The 1.75 A crystal structure of acetyl-CoA synthetase bound to adenosine-5'-propylphosphate and coenzyme A. Biochemistry 42(10):2866-2873 · Pubmed

    No abstract available.

  • Starai VJ, Takahashi H, Boeke JD, Escalante-Semerena JC (2003) Short-chain fatty acid activation by acyl-coenzyme A synthetases requires SIR2 protein function in Salmonella enterica and Saccharomyces cerevisiae. Genetics 163(2):545-555 PMC1462443 · Pubmed

    No abstract available.

  • Woodson JD, Peck RF, Krebs MP, Escalante-Semerena JC (2003) The cobY gene of the archaeon Halobacterium sp. strain NRC-1 is required for de novo cobamide synthesis. J. Bacteriol. 185(1):311-316 PMC141819 · Pubmed

    No abstract available.

  • Starai VJ, Celic I, Cole RN, Boeke JD, Escalante-Semerena JC (2002) Sir2-dependent activation of acetyl-CoA synthetase by deacetylation of active lysine. Science 298(5602):2390-2392 · Pubmed

    No abstract available.

  • Cheong CG, Escalante-Semerena JC, Rayment I (2002) Capture of a labile substrate by expulsion of water molecules from the active site of nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella enterica. J. Biol. Chem. 277(43):41120-41127 · Pubmed

    No abstract available.

  • Fonseca MV, Buan NR, Horswill AR, Rayment I, Escalante-Semerena JC (2002) The ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2'-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct. J. Biol. Chem. 277(36):33127-33131 · Pubmed

    No abstract available.

  • Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC (2002) The biosynthesis of adenosylcobalamin (vitamin B12). Nat Prod Rep 19(4):390-412 · Pubmed

    No abstract available.

  • Cheong CG, Escalante-Semerena JC, Rayment I (2002) Structural studies of the L-threonine-O-3-phosphate decarboxylase (CobD) enzyme from Salmonella enterica: the apo, substrate, and product-aldimine complexes. Biochemistry 41(29):9079-9089 · Pubmed

    No abstract available.

  • Cheong CG, Bauer CB, Brushaber KR, Escalante-Semerena JC, Rayment I (2002) Three-dimensional structure of the L-threonine-O-3-phosphate decarboxylase (CobD) enzyme from Salmonella enterica. Biochemistry 41(15):4798-808 · Pubmed

    No abstract available.

  • Horswill AR, Escalante-Semerena JC (2002) Characterization of the propionyl-CoA synthetase (PrpE) enzyme of Salmonella enterica: residue Lys592 is required for propionyl-AMP synthesis. Biochemistry 41(7):2379-2387 · Pubmed

    No abstract available.

  • Gulick AM, Horswill AR, Thoden JB, Escalante-Semerena JC, Rayment I (2002) Pentaerythritol propoxylate: a new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase. Acta Crystallogr. D Biol. Crystallogr. 58(Pt 2):306-309 · Pubmed

    No abstract available.

  • Cheong CG, Escalante-Semerena JC, Rayment I (2001) Structural investigation of the biosynthesis of alternative lower ligands for cobamides by nicotinate mononucleotide: 5,6-dimethylbenzimidazole phosphoribosyltransferase from Salmonella enterica. J. Biol. Chem. 276(40):37612-37620 · Pubmed

    No abstract available.

  • Fonseca MV, Escalante-Semerena JC (2001) An in vitro reducing system for the enzymic conversion of cobalamin to adenosylcobalamin. J. Biol. Chem. 276(34):32101-8 · Pubmed

    No abstract available.

  • Horswill AR, Dudding AR, Escalante-Semerena JC (2001) Studies of propionate toxicity in Salmonella enterica identify 2-methylcitrate as a potent inhibitor of cell growth. J. Biol. Chem. 276(22):19094-19101 · Pubmed

    No abstract available.

  • Horswill AR, Escalante-Semerena JC (2001) In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate. Biochemistry 40(15):4703-4713 · Pubmed

    No abstract available.

  • Chowdhury S, Thomas MG, Escalante-Semerena JC, Banerjee R (2001) The coenzyme b12 analog 5'-deoxyadenosylcobinamide-gdp supports catalysis by methylmalonyl-coa mutase in the absence of trans-ligand coordination. J. Biol. Chem. 276(2):1015-1019 · Pubmed

    No abstract available.

  • Bauer CB, Fonseca MV, Holden HM, Thoden JB, Thompson TB, Escalante-Semerena JC, Rayment I (2001) Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP. Biochemistry 40(2):361-74 · Pubmed

    No abstract available.

  • Thomas MG, Thompson TB, Rayment I, Escalante-Semerena JC (2000) Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation. J. Biol. Chem. 275(36):27576-27586 · Pubmed

    No abstract available.

  • Fonseca MV, Escalante-Semerena JC (2000) Reduction of Cob(III)alamin to Cob(II)alamin in Salmonella enterica serovar typhimurium LT2. J. Bacteriol. 182(15):4304-9 PMC101946 · Pubmed

    No abstract available.

  • Thomas MG, Escalante-Semerena JC (2000) Identification of an alternative nucleoside triphosphate: 5'-deoxyadenosylcobinamide phosphate nucleotidyltransferase in Methanobacterium thermoautotrophicum delta H. J. Bacteriol. 182(15):4227-33 PMC101920 · Pubmed

    No abstract available.

  • Smith JS, Brachmann CB, Celic I, Kenna MA, Muhammad S, Starai VJ, Avalos JL, Escalante-Semerena JC, Grubmeyer C, Wolberger C, Boeke JD (2000) A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family. Proc. Natl. Acad. Sci. U.S.A. 97(12):6658-6663 PMC18692 · Pubmed

    No abstract available.

  • Palacios S, Escalante-Semerena JC (2000) prpR, ntrA, and ihf functions are required for expression of the prpBCDE operon, encoding enzymes that catabolize propionate in Salmonella enterica serovar typhimurium LT2. J. Bacteriol. 182(4):905-910 PMC94363 · Pubmed

    No abstract available.

  • Downs DM, Escalante-Semerena JC (2000) Impact of genomics and genetics on the elucidation of bacterial metabolism. Methods 20(1):47-54 · Pubmed

    No abstract available.

  • Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I (1999) Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site. Biochemistry 38(40):12995-13005 · Pubmed

    No abstract available.

  • Maggio-Hall LA, Escalante-Semerena JC (1999) In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes. Proc. Natl. Acad. Sci. U.S.A. 96(21):11798-11803 PMC18366 · Pubmed

    No abstract available.

  • Horswill AR, Escalante-Semerena JC (1999) Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle. J. Bacteriol. 181(18):5615-23 PMC94080 · Pubmed

    No abstract available.

  • Horswill AR, Escalante-Semerena JC (1999) The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase. Microbiology (Reading, Engl.) 145:1381-1388 · Pubmed

    No abstract available.

  • Thomas MG, O'Toole GA, Escalante-Semerena JC (1999) Molecular characterization of eutF mutants of Salmonella typhimurium LT2 identifies eutF lesions as partial-loss-of-function tonB alleles. J. Bacteriol. 181(2):368-374 PMC93387 · Pubmed

    No abstract available.

  • Cheong CG, Escalante-Semerena JC, Rayment I (1999) The three-dimensional structures of nicotinate mononucleotide:5,6- dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella typhimurium complexed with 5,6-dimethybenzimidazole and its reaction products determined to 1.9 A resolution. Biochemistry 38(49):16125-16135 · Pubmed

    No abstract available.

  • Tsang AW, Escalante-Semerena JC (1998) CobB, a new member of the SIR2 family of eucaryotic regulatory proteins, is required to compensate for the lack of nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase activity in cobT mutants during cobalamin biosynthesis in Salmonella typhimurium LT2. J. Biol. Chem. 273(48):31788-31794 · Pubmed

    No abstract available.

  • Rondon MR, Escalante-Semerena JC (1998) High levels of transcription factor RpoS (sigma S) in mviA mutants negatively affect 1,2-propanediol-dependent transcription of the cob/pdu regulon of Salmonella typhimurium LT2. FEMS Microbiol. Lett. 169(1):147-153 · Pubmed

    No abstract available.

  • Thompson TB, Thomas MG, Escalante-Semerena JC, Rayment I (1998) Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 A resolution,. Biochemistry 37(21):7686-7695 · Pubmed

    No abstract available.

  • Brushaber KR, O'Toole GA, Escalante-Semerena JC (1998) CobD, a novel enzyme with L-threonine-O-3-phosphate decarboxylase activity, is responsible for the synthesis of (R)-1-amino-2-propanol O-2-phosphate, a proposed new intermediate in cobalamin biosynthesis in Salmonella typhimurium LT2. J. Biol. Chem. 273(5):2684-2691 · Pubmed

    No abstract available.

  • Tsang AW, Horswill AR, Escalante-Semerena JC (1998) Studies of regulation of expression of the propionate (prpBCDE) operon provide insights into how Salmonella typhimurium LT2 integrates its 1,2-propanediol and propionate catabolic pathways. J. Bacteriol. 180(24):6511-6518 PMC107752 · Pubmed

    No abstract available.

  • Trzebiatowski JR, Escalante-Semerena JC (1997) Purification and characterization of CobT, the nicotinate-mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme from Salmonella typhimurium LT2. J. Biol. Chem. 272(28):17662-17667 · Pubmed

    No abstract available.

  • Rondon MR, Trzebiatowski JR, Escalante-Semerena JC (1997) Biochemistry and molecular genetics of cobalamin biosynthesis. Prog. Nucleic Acid Res. Mol. Biol. 56:347-84 · Pubmed

    No abstract available.

  • Rondon MR, Escalante-Semerena JC (1997) Integration host factor is required for 1,2-propanediol-dependent transcription of the cob/pdu regulon in Salmonella typhimurium LT2. J. Bacteriol. 179(11):3797-3800 PMC179182 · Pubmed

    No abstract available.

  • Horswill AR, Escalante-Semerena JC (1997) Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon. J. Bacteriol. 179(3):928-940 PMC178778 · Pubmed

    No abstract available.

  • Rondon MR, Escalante-Semerena JC (1996) In vitro analysis of the interactions between the PocR regulatory protein and the promoter region of the cobalamin biosynthetic (cob) operon of Salmonella typhimurium LT2. J. Bacteriol. 178(8):2196-2203 PMC177925 · Pubmed

    No abstract available.

  • Tsang AW, Escalante-Semerena JC (1996) cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon. J. Bacteriol. 178(23):7016-7019 PMC178609 · Pubmed

    No abstract available.

  • Hammelman TA, O'Toole GA, Trzebiatowski JR, Tsang AW, Rank D, Escalante-Semerena JC (1996) Identification of a new prp locus required for propionate catabolism in Salmonella typhimurium LT2. FEMS Microbiol. Lett. 137(3-Feb):233-239 · Pubmed

    No abstract available.

  • O'Toole GA, Escalante-Semerena JC (1995) Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate. J. Biol. Chem. 270(40):23560-23569 · Pubmed

    No abstract available.

  • Rondon MR, Kazmierczak R, Escalante-Semerena JC (1995) Glutathione is required for maximal transcription of the cobalamin biosynthetic and 1,2-propanediol utilization (cob/pdu) regulon and for the catabolism of ethanolamine, 1,2-propanediol, and propionate in Salmonella typhimurium LT2. J. Bacteriol. 177(19):5434-5439 PMC177348 · Pubmed

    No abstract available.

  • Suh S, Escalante-Semerena JC (1995) Purification and initial characterization of the ATP:corrinoid adenosyltransferase encoded by the cobA gene of Salmonella typhimurium. J. Bacteriol. 177(4):921-925 PMC176684 · Pubmed

    No abstract available.

  • Rondon MR, Horswill AR, Escalante-Semerena JC (1995) DNA polymerase I function is required for the utilization of ethanolamine, 1,2-propanediol, and propionate by Salmonella typhimurium LT2. J. Bacteriol. 177(24):7119-7124 PMC177590 · Pubmed

    No abstract available.

  • O'Toole GA, Trzebiatowski JR, Escalante-Semerena JC (1994) The cobC gene of Salmonella typhimurium codes for a novel phosphatase involved in the assembly of the nucleotide loop of cobalamin. J. Biol. Chem. 269(42):26503-26511 · Pubmed

    No abstract available.

  • Trzebiatowski JR, O'Toole GA, Escalante-Semerena JC (1994) The cobT gene of Salmonella typhimurium encodes the NaMN: 5,6-dimethylbenzimidazole phosphoribosyltransferase responsible for the synthesis of N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole, an intermediate in the synthesis of the nucleotide loop of cobalamin. J. Bacteriol. 176(12):3568-3575 PMC205545 · Pubmed

    No abstract available.

  • O'Toole GA, Escalante-Semerena JC (1993) cobU-dependent assimilation of nonadenosylated cobinamide in cobA mutants of Salmonella typhimurium. J. Bacteriol. 175(19):6328-6336 PMC206730 · Pubmed

    No abstract available.

  • Suh SJ, Escalante-Semerena JC (1993) Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium. Gene 129(1):93-97 · Pubmed

    No abstract available.

  • O'Toole GA, Rondon MR, Escalante-Semerena JC (1993) Analysis of mutants of Salmonella typhimurium defective in the synthesis of the nucleotide loop of cobalamin. J. Bacteriol. 175(11):3317-3326 PMC204728 · Pubmed

    No abstract available.

  • Johnson MG, Escalante-Semerena JC (1992) Identification of 5,6-dimethylbenzimidazole as the Co alpha ligand of the cobamide synthesized by Salmonella typhimurium. Nutritional characterization of mutants defective in biosynthesis of the imidazole ring. J. Biol. Chem. 267(19):13302-13305 · Pubmed

    No abstract available.

  • Rondon MR, Escalante-Semerena JC (1992) The poc locus is required for 1,2-propanediol-dependent transcription of the cobalamin biosynthetic (cob) and propanediol utilization (pdu) genes of Salmonella typhimurium. J. Bacteriol. 174(7):2267-2672 PMC205847 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Johnson MG, Roth JR (1992) The CobII and CobIII regions of the cobalamin (vitamin B12) biosynthetic operon of Salmonella typhimurium. J. Bacteriol. 174(1):24-29 PMC205671 · Pubmed

    No abstract available.

  • O'Toole GA, Escalante-Semerena JC (1991) Identification and initial characterization of the eutF locus of Salmonella typhimurium. J. Bacteriol. 173(16):5168-5172 PMC208209 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Suh SJ, Roth JR (1990) cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium. J. Bacteriol. 172(1):273-280 PMC208428 · Pubmed

    No abstract available.

  • Hartzell PL, Escalante-Semerena JC, Bobik TA, Wolfe RS (1988) A simplified methylcoenzyme M methylreductase assay with artificial electron donors and different preparations of component C from Methanobacterium thermoautotrophicum delta H. J. Bacteriol. 170(6):2711-2715 PMC211193 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Roth JR (1987) Regulation of cobalamin biosynthetic operons in Salmonella typhimurium. J. Bacteriol. 169(5):2251-2258 PMC212145 · Pubmed

    No abstract available.

  • Hartzell PL, Zvilius G, Escalante-Semerena JC, Donnelly MI (1986) Coenzyme F420 dependence of the methylenetetrahydromethanopterin dehydrogenase of Methanobacterium thermoautotrophicum. Biochem. Biophys. Res. Commun. 133(3):884-90 · Pubmed

    No abstract available.

  • Donnelly MI, Escalante-Semerena JC, Rinehart KL, Wolfe RS (1985) Methenyl-tetrahydromethanopterin cyclohydrolase in cell extracts of Methanobacterium. Arch. Biochem. Biophys. 242(2):430-9 · Pubmed

    No abstract available.

  • RouviΗωre PE, Escalante-Semerena JC, Wolfe RS (1985) Component A2 of the methylcoenzyme M methylreductase system from Methanobacterium thermoautotrophicum. J. Bacteriol. 162(1):61-66 PMC218953 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Wolfe RS (1985) Tetrahydromethanopterin-dependent methanogenesis from non-physiological C1 donors in Methanobacterium thermoautotrophicum. J. Bacteriol. 161(2):696-701 PMC214938 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Rinehart KL, Wolfe RS (1984) Tetrahydromethanopterin, a carbon carrier in methanogenesis. J. Biol. Chem. 259(15):9447-9455 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Leigh JA, Rinehart KL, Wolfe RS (1984) Formaldehyde activation factor, tetrahydromethanopterin, a coenzyme of methanogenesis. Proc. Natl. Acad. Sci. U.S.A. 81(7):1976-1980 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Wolfe RS (1984) Formaldehyde oxidation and methanogenesis. J. Bacteriol. 158(2):721-726 PMC215489 · Pubmed

    No abstract available.

  • Escalante-Semerena JC, Blakemore RP, Wolfe RS (1980) Nitrate dissimilation under microaerophilic conditions by a magnetic spirillum. Appl. Environ. Microbiol. 40(2):429-430 · Pubmed

    No abstract available.

  • Crosby, HA et al (2012) Structural Insights into the Substrate Specificity of the Rhodopseudomonas palustris Protein Acetyltransferase RpPat. Identification of a loop critical for recognition by RpPat Journal of Biological Chemistry 287(49):41392-41404 · Pubmed

    No abstract available.

  • Moore, TC et al (2012) Structural insights into the mechanism of four-coordinate cob(II)alamin formation in the active site of the Salmonella enterica ATP:co(I)rrinoid adenosyltransferase (CobA) enzyme: Critical role of residues Phe91 and Trp93 Biochemistry 51(48):9647-9657 · Pubmed

    No abstract available.

  • Tucker, AC & Escalante-Semerena, JC (2013) Acetoacetyl-CoA synthetase activity is controlled by a protein acetyltransferase with unique domain organization in Streptomyces lividans Mol. Microbiol. 87(1):152-167 3535548 · Pubmed

    No abstract available.

  • Collins, H. et al (2013) Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species PLOS ONE 8:e55708 3573010 · Pubmed

    No abstract available.

  • Chan, CH (2014) Dissecting cobamide diversity through structural and functional analyses of the base-activating CobT enzyme of Salmonella enterica Biophysica et Biochimica Acta 1840(1):464-475 · Pubmed

    No abstract available.

  • Moore, T C, PE Mera, & JC Escalante-Semerena (2014) The EutT enzyme of Salmonella enterica is a unique ATP:Cob(I)alamin adenosyltransferase metalloprotein that requires ferrous ions for maximal activity J. Bacteriol. 196(4):903-910 · Pubmed

    No abstract available.

  • Crosby, HA & JC Escalante-Semerena (2014) The acetylation motif in AMP-forming acyl-CoA synthetases contains residues critical for acetylation and recognition by the protein acetyltransferase Pat of Rhodopseudomonas palustris J. Bacteriol. 196(8):1496-1504 4053389 · Pubmed

    No abstract available.

  • Tucker, AC, & JC Escalante-Semerena (2014) Determinants within the C-terminal domain of Streptomyces lividans acetyl-CoA synthetase that block acetylation of its active site lysine in vitro by the protein acetyltransferase (Pat) enzyme PLOS ONE 9(6):e99817 · Pubmed

    No abstract available.

  • You, D, L-L Yao, D Huang, JC Escalante-Semerena, B-C Ye (2014) Acetyl-CoA synthetase is acetylated on multiple lysine residues by a protein acetyltransferase with single GNAT domain in Saccharopolyspora erythraea Journal of Bacteriology 196(17):3169-317824957627 · Pubmed

    No abstract available.

  • Pallares, IG, TC Moore, JC Escalante-Semerena, TC Brunold (2014) Sprectoscopic studies of the Salmonella enterica adenosyltransferase enzyme SeCobA: Molecular insight into the mechanism of substrate cob(II)alamin activation Biochemistry 53:7969-7982 · Pubmed

    No abstract available.

  • Tucker, A. C., K. A. Taylor, K. C. Rank, I. Rayment, and J. C. Escalante-Semerena (2014) Insights into the specificity of lysine acetyltransferases J. Biol. Chem. 289(52):36249-26262 PMC4276886 · Pubmed

    No abstract available.

  • Hentchel, K. L., and J. C. Escalante-Semerena (2015) In Salmonella enterica, the Gcn5-related acetyltransferase MddA (formerly YncA) acetylates methionine sulfoximine and methionine sulfone, blocking their toxic effects J. Bacteriol. 197(2):314-325 · Pubmed

    No abstract available.

  • Mitchell, C. A., A. C. Tucker, J. C. Escalante-Semerena, and A. M. Gulick (2015) The structure of S. lividans acetoacetyl-CoA synthetase shows a novel interaction between the C-terminal extension and the N-terminal domain Proteins 83(3):575-581 PMC4329092 · Pubmed

    No abstract available.

  • Park, K., P. E. Mera, T. C. Moore, J. C. Escalante-Semerena, and T. C. Brunold (2015) Unprecedented mechanism employed by the Salmonella enterica EutT ATP:Co(I)rrinoid adenosyltransferase precludes adenosylation of incomplete Co(II)rrinoids Angewandte Chemie 54(24):7158-7161 4504680 · Pubmed

    No abstract available.

  • Hentchel, K. L., and J. C. Escalante-Semerena (2015) Acylation of biomolecules in prokaryotes: a widespread strategy for the control of biological function and metabolic stress Miceobiology and Molecular Biology Reviews 79(3):321-346 10.1128/MMBR.00020-15 · Pubmed

    No abstract available.

  • Hentchel, K.L., S. Thao, P. J. Intile, and J. C. Escalante-Semerena (2015) Deciphering the regulatory circuitry that controls reversible lysine acetylation in Salmonella enterica mBio 6(4):e00891-15 4513076 10.1128/mBio.00891-15 · Pubmed

    No abstract available.

  • Stuecker, T. N., S. Bramhacharya, K. M. Hodge-Hanson, G. Suen, J. C. Escalante-Semerena (2015) Phylogenetic and amino acid conservation analyses of bacterial L-aspartate-Χρ-decarboxylase and of its zymogen-maturation protein reveal a putative interaction domain BMC Research Notes 8:354 10.1186/s13104-015-1314-6 · DOI

    No abstract available.